Journal: Cell Reports Medicine

Article Title: Nanrilkefusp alfa (SOT101), an IL-15 receptor βγ superagonist, as a single agent or with anti-PD-1 in patients with advanced cancers

doi: 10.1016/j.xcrm.2025.101967

Figure Lengend Snippet:

Article Snippet: RNeasy MinElute Cleanup Kit , Qiagen , Cat#74204.

Techniques: Polymer, Plasmid Preparation, Recombinant, Staining, Lysis, Software

Journal: Nucleic Acids Research

Article Title: Transcription factor Zfp276 drives oligodendroglial differentiation and myelination by switching off the progenitor cell program

doi: 10.1093/nar/gkac042

Figure Lengend Snippet: Forced expression of Zfp276 in 1° rat OGCs induces premature differentiation. 1° rat oligodendroglial cells (OGCs) were transduced with an EGFP only virus (ctrl, white bars) or a Zfp276-IRES-EGFP virus (Zfp276oe, black bars). ( A ) Quantification of transduction rates as percent of EGFP/Sox10-double-positive OGCs per total number of Sox10-positive cells ( B, C, F, H ). Quantification of O4- (B), Mbp- (C, F) or Pdgfra- (H) positive oligodendrocytes (OLs) of ctrl or Zfp276oe 1° rat OGCs at 3 or 6 days of differentiation (3ddiff: B-C, H, 6ddiff: F). Number of O4-, Mbp- or Pdgfra-positive transduced OGCs was divided by the total number of Sox10-positive cells and the mean value of the control set to 100% (mean values ± SEM). ( D, E, I, J ) Representative images of 3ddiff (D, E) and 6ddiff (I, J) 1° rat OLs stained for O4 (magenta, D, E) or Mbp (magenta, I, J), EGFP (green) and Sox10 (cyan), transduced with a ctrl (D, I) or Zfp276oe (E, J) retrovirus; scale bar: 50 μm. ( G ) Cell process morphology of transduced OGCs after 6ddiff and stained for Mbp was assessed using an ImageJ software plugin for Sholl analysis and the ramification index of ctrl cells was set to 100% (mean values ± SEM). ( K–M ) Quantification of Mbp- (K), phosphohistone-3 (pH3)- (L) or cleaved Caspase3 (cCasp3)- (M) positive OGCs after 3 days in proliferating conditions (3dprol). Number of marker-positive OGCs were divided by the total number of Sox10-positive cells and the mean value of the control was set to 100% (mean values ± SEM). ( N ) qRT-PCR was performed on total RNA isolated from virally transduced and FACS-purified oligodendroglial ctrl and Zfp276oe CG4 cells using specific oligonucleotides for transcripts of immaturity markers Id2, Id4, Tgfb2, Cspg4 and Pdgfra . Expression levels were normalized to Gapdh and Rpl8 . Ctrl values were arbitrarily set to 1 and relative expression was presented as mean values ± SEM. Statistical analysis was performed with Student’s two-tailed t -test (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001).

Article Snippet: Crosslinking was reversed via incubation at 65°C for 2 h with proteinase K digestion (Roche, 03115844001), and DNA was purified with the NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel).

Techniques: Expressing, Transduction, Virus, Control, Staining, Software, Marker, Quantitative RT-PCR, Isolation, Purification, Two Tailed Test

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ on native human saliva samples alter protein yield. Representaive 2D gel electrophoresis results of untreated ( a ) and ReadyPrep 2-D Cleanup kit™, ReadyPrep Reduction-Alkylation kit™ treated ( b ) native human saliva samples (no RNAlater™ treatment) demonstrate both procedures alter protein yield primarily at the low molecular range. Highlighted areas (1–3) indicate identical regions of the separation. Proteins were visualized by silver stain.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Impact of reduction-alkylation during 2D gel electrophoresis of native saliva samples. Representative 2D gel electrophoresis results on human saliva samples with the use of ReadyPrep 2-D Cleanup kit™ ( a ) in comparison with ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ ( b ) treated samples indicate Reduction-Alkylation kit™ slightly improves spot detection and resolution. Arrowheads highlight visible differences in spot resolution and protein yield quality.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Comparison

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Altered protein yields of RNAlater™ containing saliva samples following ReadyPrep 2D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ treatments in comparison with untreated, native saliva samples. 2D gel electrophoresis results of a representative untreated human saliva ( a ) compared with RNAlater™, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ treated sample ( b ) indicate a significant decrease in protein yields despite of the protective manipulations. Highlighted areas (1–3) represent identical protein regions following separation.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Comparison, Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Application of ReadyPrep Reduction-Alkylation kit™ does not improve the quality 2D gel electrophoresis of human saliva samples when pretreated with RNAlater™. Representative 2D gel electrophoresis of human selectively precipitated saliva samples with RNAlater™ ( a ) in relation with saliva sample treated with RNAlater™, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ ( b ) demonstrate lack of significant separation improvement with RA (Black arrows point at identical proteins on a and b).

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Impact of RNAlater™ on 2D gel electrophoresis of saliva samples treated with both, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™. The addition of RNAlater™ to precipitated and reduction-alkylated protein samples demonstrates a significant reduction of protein yield and quality following 2D separation ( b ) when compared to saliva without RNAlater™ ( a ). Numbers 1–3 correspond to similar regions of interest.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis